This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakajima, H. Articles by Aono, R. Search for Related Content PubMed PubMed Citation Articles by Nakajima, H. Articles by Aono, R. Agricola Articles by Nakajima, H. Articles by Aono, R.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 06 1995, 2302-2307, Vol 61, No. 6
Copyright © 1995, American Society for Microbiology

Overexpression of the robA gene increases organic solvent tolerance and multiple antibiotic and heavy metal ion resistance in Escherichia coli

H Nakajima, K Kobayashi, M Kobayashi, H Asako and R Aono
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.

Escherichia coli K-12 OST3410 was isolated previously as a stable cyclohexane-tolerant mutant derived from cyclohexane-sensitive strain JA300. A plasmid which provides cyclohexane tolerance to strain JA300 was isolated from the OST3410 genomic library. Subcloning and sequence analysis showed that the plasmid contained the robA gene, whose gene product was reported to bind specifically to the right border of oriC. We observed that the robA gene on the multicopy plasmid generally increased the organic solvent tolerance of several E. coli strains. We also observed an increase in the organic solvent tolerance of JA300 carrying the lac-robA fusion gene on a low-copy plasmid by isopropyl- beta-D-thiogalactopyranoside induction. Strain JA300 carrying the multicopy robA plasmid also showed an increase in resistance to a number of unrelated antibiotics and heavy metal ions, and the spectrum of resistance was significantly similar to that of the soxS- overexpressing strain.

This article has been cited by other articles:

• Barchiesi, J., Castelli, M. E., Soncini, F. C., Vescovi, E. G. (2008). mgtA Expression Is Induced by Rob Overexpression and Mediates a Salmonella enterica Resistance Phenotype. J. Bacteriol. 190: 4951-4958 [Abstract] [Full Text]  
• Matsui, K., Teranishi, S., Kamon, S., Kuroda, K., Ueda, M. (2008). Discovery of a Modified Transcription Factor Endowing Yeasts with Organic-Solvent Tolerance and Reconstruction of an Organic-Solvent-Tolerant Saccharomyces cerevisiae Strain. Appl. Environ. Microbiol. 74: 4222-4225 [Abstract] [Full Text]  
• Schneiders, T., Levy, S. B. (2006). MarA-mediated Transcriptional Repression of the rob Promoter. J. Biol. Chem. 281: 10049-10055 [Abstract] [Full Text]  
• Shimizu, K., Hayashi, S., Kako, T., Suzuki, M., Tsukagoshi, N., Doukyu, N., Kobayashi, T., Honda, H. (2005). Discovery of glpC, an Organic Solvent Tolerance-Related Gene in Escherichia coli, Using Gene Expression Profiles from DNA Microarrays. Appl. Environ. Microbiol. 71: 1093-1096 [Abstract] [Full Text]  
• Abe, S., Okutsu, T., Nakajima, H., Kakuda, N., Ohtsu, I., Aono, R. (2003). n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane. Microbiology 149: 1265-1273 [Abstract] [Full Text]  
• Rosner, J. L., Dangi, B., Gronenborn, A. M., Martin, R. G. (2002). Posttranscriptional Activation of the Transcriptional Activator Rob by Dipyridyl in Escherichia coli. J. Bacteriol. 184: 1407-1416 [Abstract] [Full Text]  
• Wang, H., Dzink-Fox, J. L., Chen, M., Levy, S. B. (2001). Genetic Characterization of Highly Fluoroquinolone-Resistant Clinical Escherichia coli Strains from China: Role of acrR Mutations. Antimicrob. Agents Chemother. 45: 1515-1521 [Abstract] [Full Text]  
• Kobayashi, K., Tsukagoshi, N., Aono, R. (2001). Suppression of Hypersensitivity of Escherichia coli acrB Mutant to Organic Solvents by Integrational Activation of the acrEF Operon with the IS1 or IS2 Element. J. Bacteriol. 183: 2646-2653 [Abstract] [Full Text]  
• Wei, Y., Vollmer, A. C., LaRossa, R. A. (2001). In Vivo Titration of Mitomycin C Action by Four Escherichia coli Genomic Regions on Multicopy Plasmids. J. Bacteriol. 183: 2259-2264 [Abstract] [Full Text]  
• Bennik, M. H. J., Pomposiello, P. J., Thorne, D. F., Demple, B. (2000). Defining a rob Regulon in Escherichia coli by Using Transposon Mutagenesis. J. Bacteriol. 182: 3794-3801 [Abstract] [Full Text]  
• Asako, H., Kobayashi, K., Aono, R. (1999). Organic Solvent Tolerance of Escherichia coli Is Independent of OmpF Levels in the Membrane. Appl. Environ. Microbiol. 65: 294-296 [Abstract] [Full Text]  
• McDonnell, G., Russell, A. D. (1999). Antiseptics and Disinfectants: Activity, Action, and Resistance. Clin. Microbiol. Rev. 12: 147-179 [Abstract] [Full Text]  
• Oethinger, M., Podglajen, I., Kern, W. V., Levy, S. B. (1998). Overexpression of the marA or soxS Regulatory Gene in Clinical Topoisomerase Mutants of Escherichia coli. Antimicrob. Agents Chemother. 42: 2089-2094 [Abstract] [Full Text]  
• Li, X.-Z., Zhang, L., Poole, K. (1998). Role of the Multidrug Efflux Systems of Pseudomonas aeruginosa in Organic Solvent Tolerance. J. Bacteriol. 180: 2987-2991 [Abstract] [Full Text]  
• Aono, R., Tsukagoshi, N., Yamamoto, M. (1998). Involvement of Outer Membrane Protein TolC, a Possible Member of the mar-sox Regulon, in Maintenance and Improvement of Organic Solvent Tolerance of Escherichia coli K-12. J. Bacteriol. 180: 938-944 [Abstract] [Full Text]  
• Kieboom, J., Dennis, J. J., de Bont, J. A. M., Zylstra, G. J. (1998). Identification and Molecular Characterization of an Efflux Pump Involved in Pseudomonas putida S12 Solvent Tolerance. J. Biol. Chem. 273: 85-91 [Abstract] [Full Text]