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Appl. Environ. Microbiol., Jun 1995, 2159-2165, Vol 61, No. 6
Copyright © 1995, American Society for Microbiology

Formation of Dimethylmuconolactones from Dimethylphenols by Alcaligenes eutrophus JMP 134

DH Pieper, K Stadler-Fritzsche, H Knackmuss and KN Timmis
Bereich Mikrobiologie, Gesellschaft fur Biotechnologische Forschung, 38124 Braunschweig, and Institut fur Mikrobiologie der Universitat Stuttgart, 70569 Stuttgart, Germany

2,3-, 2,4-, 2,5-, 3,4-, and 3,5-dimethylphenols were cometabolized by 2,4-dichlorophenoxyacetate-grown Alcaligenes eutrophus JMP 134 or the constitutive derivative JMP 134-1 via the ortho pathway into dimethylmuconolactones as dead-end products. Formation of two distinct lactones from 3,4-dimethylphenol is indicative of 2- as well as 6-hydroxylation. Induction of the meta-cleavage pathway by 2,3- and 3,4-dimethylphenols resulted in growth and no accumulation of products. In contrast, 3,5-dimethylphenol is not metabolized by the meta-cleavage pathway.

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