This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lovley, D. R. Articles by Widman, P. K. Search for Related Content PubMed PubMed Citation Articles by Lovley, D. R. Articles by Widman, P. K. Agricola Articles by Lovley, D. R. Articles by Widman, P. K.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 06 1995, 2132-2138, Vol 61, No. 6
Copyright © 1995, American Society for Microbiology

Fe(III) and S0 reduction by Pelobacter carbinolicus

DR Lovley, EJ Phillips, DJ Lonergan and PK Widman

There is a close phylogenetic relationship between Pelobacter species and members of the genera Desulfuromonas and Geobacter, and yet there has been a perplexing lack of physiological similarities. Pelobacter species have been considered to have a fermentative metabolism. In contrast, Desulfuromonas and Geobacter species have a respiratory metabolism with Fe(III) serving as the common terminal electron acceptor in all species. However, the ability of Pelobacter species to reduce Fe(III) had not been previously evaluated. When a culture of Pelobacter carbinolicus that had grown by fermentation of 2,3- butanediol was inoculated into the same medium supplemented with Fe(III), the Fe(III) was reduced. There was less accumulation of ethanol and more production of acetate in the presence of Fe(III). P. carbinolicus grew with ethanol as the sole electron donor and Fe(III) as the sole electron acceptor. Ethanol was metabolized to acetate. Growth was also possible on Fe(III) with the oxidation of propanol to propionate or butanol to butyrate if acetate was provided as a carbon source. P. carbinolicus appears capable of conserving energy to support growth from Fe(III) respiration as it also grew with H2 or formate as the electron donor and Fe(III) as the electron acceptor. Once adapted to Fe(III) reduction, P. carbinolicus could also grow on ethanol or H2 with S0 as the electron acceptor. P. carbinolicus did not contain detectable concentrations of the c-type cytochromes that previous studies have suggested are involved in electron transport to Fe(III) in other organisms that conserve energy to support growth from Fe(III) reduction.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

• Risso, C., Methe, B. A., Elifantz, H., Holmes, D. E., Lovley, D. R. (2008). Highly conserved genes in Geobacter species with expression patterns indicative of acetate limitation. Microbiology 154: 2589-2599 [Abstract] [Full Text]  
• Haveman, S. A., DiDonato, R. J. Jr., Villanueva, L., Shelobolina, E. S., Postier, B. L., Xu, B., Liu, A., Lovley, D. R. (2008). Genome-Wide Gene Expression Patterns and Growth Requirements Suggest that Pelobacter carbinolicus Reduces Fe(III) Indirectly via Sulfide Production. Appl. Environ. Microbiol. 74: 4277-4284 [Abstract] [Full Text]  
• Omoregie, E. O., Mastalerz, V., de Lange, G., Straub, K. L., Kappler, A., Roy, H., Stadnitskaia, A., Foucher, J.-P., Boetius, A. (2008). Biogeochemistry and Community Composition of Iron- and Sulfur-Precipitating Microbial Mats at the Chefren Mud Volcano (Nile Deep Sea Fan, Eastern Mediterranean). Appl. Environ. Microbiol. 74: 3198-3215 [Abstract] [Full Text]  
• Holmes, D. E., Mester, T., O'Neil, R. A., Perpetua, L. A., Larrahondo, M. J., Glaven, R., Sharma, M. L., Ward, J. E., Nevin, K. P., Lovley, D. R. (2008). Genes for two multicopper proteins required for Fe(III) oxide reduction in Geobacter sulfurreducens have different expression patterns both in the subsurface and on energy-harvesting electrodes. Microbiology 154: 1422-1435 [Abstract] [Full Text]  
• Richter, H., Lanthier, M., Nevin, K. P., Lovley, D. R. (2007). Lack of Electricity Production by Pelobacter carbinolicus Indicates that the Capacity for Fe(III) Oxide Reduction Does Not Necessarily Confer Electron Transfer Ability to Fuel Cell Anodes. Appl. Environ. Microbiol. 73: 5347-5353 [Abstract] [Full Text]  
• Haveman, S. A., Holmes, D. E., Ding, Y.-H. R., Ward, J. E., DiDonato, R. J. Jr., Lovley, D. R. (2006). c-Type Cytochromes in Pelobacter carbinolicus. Appl. Environ. Microbiol. 72: 6980-6985 [Abstract] [Full Text]  
• Moreira, D., Rodriguez-Valera, F., Lopez-Garcia, P. (2006). Metagenomic analysis of mesopelagic Antarctic plankton reveals a novel deltaproteobacterial group. Microbiology 152: 505-517 [Abstract] [Full Text]  
• Feinberg, L. F., Holden, J. F. (2006). Characterization of Dissimilatory Fe(III) versus NO3- Reduction in the Hyperthermophilic Archaeon Pyrobaculum aerophilum. J. Bacteriol. 188: 525-531 [Abstract] [Full Text]  
• Metje, M., Frenzel, P. (2005). Effect of Temperature on Anaerobic Ethanol Oxidation and Methanogenesis in Acidic Peat from a Northern Wetland. Appl. Environ. Microbiol. 71: 8191-8200 [Abstract] [Full Text]  
• Holmes, D. E., Nevin, K. P., Lovley, D. R. (2004). Comparison of 16S rRNA, nifD, recA, gyrB, rpoB and fusA genes within the family Geobacteraceae fam. nov.. Int. J. Syst. Evol. Microbiol. 54: 1591-1599 [Abstract] [Full Text]  
• King, G. M., Garey, M. A. (1999). Ferric Iron Reduction by Bacteria Associated with the Roots of Freshwater and Marine Macrophytes. Appl. Environ. Microbiol. 65: 4393-4398 [Abstract] [Full Text]  
• Kieft, T. L., Fredrickson, J. K., Onstott, T. C., Gorby, Y. A., Kostandarithes, H. M., Bailey, T. J., Kennedy, D. W., Li, S. W., Plymale, A. E., Spadoni, C. M., Gray, M. S. (1999). Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a Thermus Isolate. Appl. Environ. Microbiol. 65: 1214-1221 [Abstract] [Full Text]