This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bourbonnais, R. Articles by Yaguchi, M. Search for Related Content PubMed PubMed Citation Articles by Bourbonnais, R. Articles by Yaguchi, M. Agricola Articles by Bourbonnais, R. Articles by Yaguchi, M.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., May 1995, 1876-1880, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization

R Bourbonnais, MG Paice, ID Reid, P Lanthier and M Yaguchi
Pulp and Paper Research Institute of Canada, Pointe Claire, Quebec, Ontario.

Two laccase isozymes (I and II) produced by the white-rot fungus Trametes versicolor were purified, and their reactivities towards various substrates and lignins were studied. The N-terminal amino acid sequences of these enzymes were determined and compared to other known laccase sequences. Laccase II showed a very high sequence similarity to a laccase which was previously reported to depolymerize lignin. The reactivities of the two isozymes on most of the substrates tested were similar, but there were some differences in the oxidation rate of polymeric substrates. We found that the two laccases produced similar qualitative effects on kraft lignin and residual lignin in kraft pulp, with no evidence of a marked preference for depolymerization by either enzyme. However, the presence of the mediator 2,2'-azinobis(3- ethylbenzthiazoline-6-sulfonate) prevented and reversed the polymerization of kraft lignin by either laccase. The delignification of hardwood and softwood kraft pulps with the two isozymes and the mediator was compared; either laccase was able to reduce the kappa number of pulp, but only in the presence of 2,2'-azinobis(3- ethylbenzthiazoline-6-sulfonate).

This article has been cited by other articles:

• Tauber, M. M., Guebitz, G. M., Rehorek, A. (2005). Degradation of Azo Dyes by Laccase and Ultrasound Treatment. Appl. Environ. Microbiol. 71: 2600-2607 [Abstract] [Full Text]  
• Alves, A. M. C. R., Record, E., Lomascolo, A., Scholtmeijer, K., Asther, M., Wessels, J. G. H., Wosten, H. A. B. (2004). Highly Efficient Production of Laccase by the Basidiomycete Pycnoporus cinnabarinus. Appl. Environ. Microbiol. 70: 6379-6384 [Abstract] [Full Text]  
• Enguita, F. J., Marcal, D., Martins, L. O., Grenha, R., Henriques, A. O., Lindley, P. F., Carrondo, M. A. (2004). Substrate and Dioxygen Binding to the Endospore Coat Laccase from Bacillus subtilis. J. Biol. Chem. 279: 23472-23476 [Abstract] [Full Text]  
• Xiao, Y. Z., Chen, Q., Hang, J., Shi, Y. Y., Xiao, Y. Z., Wu, J., Hong, Y. Z., Wang, Y. P. (2004). Selective induction, purification and characterization of a laccase isozyme from the basidiomycete Trametes sp. AH28-2. Mycologia 96: 26-35 [Abstract] [Full Text]  
• Gonzalez, T., Terron, M. C., Zapico, E. J., Tellez, A., Yague, S., Carbajo, J. M., Gonzalez, A. E. (2003). Use of Multiplex Reverse Transcription-PCR To Study the Expression of a Laccase Gene Family in a Basidiomycetous Fungus. Appl. Environ. Microbiol. 69: 7083-7090 [Abstract] [Full Text]  
• Mansur, M., Arias, M. E., Copa-Patino, J. L., Flardh, M., Gonzalez, A. E. (2003). The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities. Mycologia 95: 1013-1020 [Abstract] [Full Text]  
• Alcalde, M., Bulter, T., Arnold, F. H. (2002). Colorimetric Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal Laccases. J Biomol Screen 7: 547-553 [Abstract]  
• Soden, D. M., O'Callaghan, J., Dobson, A. D. W. (2002). Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host. Microbiology 148: 4003-4014 [Abstract] [Full Text]  
• Levin, L., Forchiassin, F., Ramos, A. M. (2002). Copper induction of lignin-modifying enzymes in the white-rot fungus Trametes trogii. Mycologia 94: 377-383 [Abstract] [Full Text]  
• Saparrat, M. C. N., Guillen, F., Arambarri, A. M., Martinez, A. T., Martinez, M. J. (2002). Induction, Isolation, and Characterization of Two Laccases from the White Rot Basidiomycete Coriolopsis rigida. Appl. Environ. Microbiol. 68: 1534-1540 [Abstract] [Full Text]  
• Gouka, R. J., van der Heiden, M., Swarthoff, T., Verrips, C. T. (2001). Cloning of a Phenol Oxidase Gene from Acremonium murorum and Its Expression in Aspergillus awamori. Appl. Environ. Microbiol. 67: 2610-2616 [Abstract] [Full Text]  
• Abadulla, E., Tzanov, T., Costa, S., Robra, K.-H., Cavaco-Paulo, A., Gübitz, G. M. (2000). Decolorization and Detoxification of Textile Dyes with a Laccase from Trametes hirsuta. Appl. Environ. Microbiol. 66: 3357-3362 [Abstract] [Full Text]  
• Dedeyan, B., Klonowska, A., Tagger, S., Tron, T., Iacazio, G., Gil, G., Le Petit, J. (2000). Biochemical and Molecular Characterization of a Laccase from Marasmius quercophilus. Appl. Environ. Microbiol. 66: 925-929 [Abstract] [Full Text]  
• Johannes, C., Majcherczyk, A. (2000). Natural Mediators in the Oxidation of Polycyclic Aromatic Hydrocarbons by Laccase Mediator Systems. Appl. Environ. Microbiol. 66: 524-528 [Abstract] [Full Text]  
• Gelo-Pujic, M., Kim, H.-H., Butlin, N. G., Palmore, G. T. R. (1999). Electrochemical Studies of a Truncated Laccase Produced in Pichia pastoris. Appl. Environ. Microbiol. 65: 5515-5521 [Abstract] [Full Text]  
• Zhao, J., Kwan, H. S. (1999). Characterization, Molecular Cloning, and Differential Expression Analysis of Laccase Genes from the Edible Mushroom Lentinula edodes. Appl. Environ. Microbiol. 65: 4908-4913 [Abstract] [Full Text]  
• Yaver, D. S., Overjero, M. D. C., Xu, F., Nelson, B. A., Brown, K. M., Halkier, T., Bernauer, S., Brown, S. H., Kauppinen, S. (1999). Molecular Characterization of Laccase Genes from the Basidiomycete Coprinus cinereus and Heterologous Expression of the Laccase Lcc1. Appl. Environ. Microbiol. 65: 4943-4948 [Abstract] [Full Text]  
• Collins, P. J., Dobson, A. D. W., Field, J. A. (1998). Reduction of the 2,2'-Azinobis(3-Ethylbenzthiazoline-6-Sulfonate) Cation Radical by Physiological Organic Acids in the Absence and Presence of Manganese. Appl. Environ. Microbiol. 64: 2026-2031 [Abstract] [Full Text]  
• Reid, I. D. (1998). Fate of Residual Lignin during Delignification of Kraft Pulp by Trametes versicolor. Appl. Environ. Microbiol. 64: 2117-2125 [Abstract] [Full Text]  
• Mansur, M., Suárez, T., González, A. E. (1998). Differential Gene Expression in the Laccase Gene Family from Basidiomycete I-62 (CECT 20197). Appl. Environ. Microbiol. 64: 771-774 [Abstract] [Full Text]