This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rothschild, N. Articles by Dosoretz, C. Search for Related Content PubMed PubMed Citation Articles by Rothschild, N. Articles by Dosoretz, C. Agricola Articles by Rothschild, N. Articles by Dosoretz, C.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., May 1995, 1833-1838, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Ligninolytic System Formation by Phanerochaete chrysosporium in Air

N Rothschild, Y Hadar and C Dosoretz
MIGAL, Galilee Technological Center, Kiryat Shmona 10200; Department of Plant Pathology & Microbiology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76200; and Department of Environmental Engineering and Water Resources, Technion-Israel Institute of Technology, Haifa 32000, Israel

This study characterizes the effect of oxygen concentration on the synthesis of ligninolytic enzymes by Phanerochaete chrysosporium immobilized on polyurethane foam cubes in a nonimmersed liquid culture system and maintained under different carbon-to-nitrogen (C/N) ratios and levels. Lignin peroxidase (LIP) activity was obtained in cultures exposed to air when the C/N ratio was low (7.47), i.e., when nitrogen levels were high (C/N = 56/45 mM) or carbon levels were low (C/N = 5.6/4.5 mM). At the low C/N ratio, the fungus was carbon starved and did not produce extracellular polysaccharides. At a high C/N ratio (153), i.e., under conditions of excess carbon (nitrogen limitation) (C/N = 56/2.2 mM), cultures exposed to air produced large amounts of polysaccharide, and LIP activity was detected only in cultures exposed to pure oxygen. Under high-nitrogen conditions, LIP production was 1,800 U/liter in cultures exposed to pure oxygen and 1,300 U/liter in cultures exposed to air, with H1 and H2 being the main isoenzymes. The oxygen level did not significantly alter the isoenzyme profile, nor did low-carbon conditions. The formation of manganese peroxidase was generally less affected by the oxygen level than that of LIP but was considerably reduced by a low C/N ratio. The effects of oxygen level and C/N ratio on the synthesis of glyoxal oxidase paralleled their effects on LIP synthesis except in the case of high nitrogen, which totally suppressed glyoxal oxidase activity.

This article has been cited by other articles:

• Matityahu, A., Hadar, Y., Dosoretz, C. G., Belinky, P. A. (2008). Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium. Appl. Environ. Microbiol. 74: 5359-5365 [Abstract] [Full Text]  
• Belinky, P. A., Flikshtein, N., Lechenko, S., Gepstein, S., Dosoretz, C. G. (2003). Reactive Oxygen Species and Induction of Lignin Peroxidase in Phanerochaete chrysosporium. Appl. Environ. Microbiol. 69: 6500-6506 [Abstract] [Full Text]  
• Rothschild, N., Levkowitz, A., Hadar, Y., Dosoretz, C. G. (1999). Manganese Deficiency Can Replace High Oxygen Levels Needed for Lignin Peroxidase Formation by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 65: 483-488 [Abstract] [Full Text]