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Appl. Environ. Microbiol., May 1995, 1688-1690, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Randomly amplified polymorphic DNA analysis of Xylella fastidiosa Pierce's disease and oak leaf scorch pathotypes

J Chen, O Lamikanra, CJ Chang and DL Hopkins
Center for Viticultural Science, Florida A&M University, Tallahassee 32307, USA.

Randomly amplified polymorphic DNA analysis was conducted with 14 primers to 17 strains of Xylella fastidiosa. There was a high degree of similarity among the seven Pierce's disease (PD) strains (Sxy > 0.93) and the seven oak leaf scorch (OLS) strains (Sxy > 0.96). However, the two groups were different, with a similarity index of 0.67, confirming the presence of a PD DNA cluster and suggesting the presence of a new OLS cluster. The control plum leaf scald strains (two strains) together with the periwinkle wilt strain had a much smaller similarity index (0.44) compared with the PD and OLS clusters.


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