This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilson, M. Articles by Lindow, S. E. Search for Related Content PubMed PubMed Citation Articles by Wilson, M. Articles by Lindow, S. E. Agricola Articles by Wilson, M. Articles by Lindow, S. E.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Mar 1995, 1073-1076, Vol 61, No. 3
Copyright © 1995, American Society for Microbiology

Enhanced Epiphytic Coexistence of Near-Isogenic Salicylate-Catabolizing and Non-Salicylate-Catabolizing Pseudomonas putida Strains after Exogenous Salicylate Application

M Wilson and SE Lindow
Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720

The hypothesis that epiphytic bacterial populations can coexist through nutritional resource partitioning was tested with the near-isogenic bacterial strain pair Pseudomonas putida R20 and R20(pNAH7). The plasmid pNAH7 conferred upon R20 the ability to catabolize salicylate as a sole carbon source in vitro. P. putida R20(pNAH7) also catabolized exogenously applied salicylate in planta and reached a significantly larger epiphytic population size than the near-isogenic parental strain R20 under the same conditions. This supports previous observations that epiphytic populations on plants grown under nitrogen-sufficient conditions are limited by carbon availability. In the absence of exogenous salicylate, R20 and R20(pNAH7) competed for and partitioned endogenous carbon according to their inoculum proportion in replacement series experiments, exhibiting a low level of coexistence. In the presence of exogenous salicylate, however, R20(pNAH7) was solely able to catabolize the additional carbon and achieved a higher level of coexistence with R20 than was possible in the absence of exogenous carbon.

This article has been cited by other articles:

• Ji, P., Wilson, M. (2003). Enhancement of Population Size of a Biological Control Agent and Efficacy in Control of Bacterial Speck of Tomato through Salicylate and Ammonium Sulfate Amendments. Appl. Environ. Microbiol. 69: 1290-1294 [Abstract] [Full Text]  
• Watanabe, K., Miyashita, M., Harayama, S. (2000). Starvation Improves Survival of Bacteria Introduced into Activated Sludge. Appl. Environ. Microbiol. 66: 3905-3910 [Abstract] [Full Text]  
• Ghiglione, J.-F., Gourbiere, F., Potier, P., Philippot, L., Lensi, R. (2000). Role of Respiratory Nitrate Reductase in Ability of Pseudomonas fluorescens YT101 To Colonize the Rhizosphere of Maize. Appl. Environ. Microbiol. 66: 4012-4016 [Abstract] [Full Text]  
• Mercier, J., Lindow, S. E. (2000). Role of Leaf Surface Sugars in Colonization of Plants by Bacterial Epiphytes. Appl. Environ. Microbiol. 66: 369-374 [Abstract] [Full Text]  
• Gardener, B. B. M., de Bruijn, F. J. (1998). Detection and Isolation of Novel Rhizopine-Catabolizing Bacteria from the Environment. Appl. Environ. Microbiol. 64: 4944-4949 [Abstract] [Full Text]