This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arensdorf, J. J. Articles by Focht, D. D. Search for Related Content PubMed PubMed Citation Articles by Arensdorf, J. J. Articles by Focht, D. D. Agricola Articles by Arensdorf, J. J. Articles by Focht, D. D.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Feb 1995, 443-447, Vol 61, No. 2
Copyright © 1995, American Society for Microbiology

A meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166

JJ Arensdorf and DD Focht
Environmental Toxicology Graduate Program, University of California, Riverside 92521, USA.

Bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2- hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). Pseudomonas cepacia P166 utilizes 4-chlorobiphenyl for growth and produces 4-chlorobenzoate as a central intermediate. In this study we found that strain P166 further transforms 4-chlorobenzoate to 4-chlorocatechol, which is mineralized by a meta cleavage pathway. Key metabolites which we identified include the meta cleavage product (5-chloro-2-hydroxymuconic semialdehyde), 5-chloro-2-hydroxymuconate, 5-chloro-2-oxopent-4-enoate, 5-chloro-4-hydroxy-2-oxopentanoate, and chloroacetate. Chloroacetate accumulated transiently, and slow but stoichiometric dehalogenation was observed.

This article has been cited by other articles:

• Pollmann, K., Kaschabek, S., Wray, V., Reineke, W., Pieper, D. H. (2002). Metabolism of Dichloromethylcatechols as Central Intermediates in the Degradation of Dichlorotoluenes by Ralstonia sp. Strain PS12. J. Bacteriol. 184: 5261-5274 [Abstract] [Full Text]  
• Katsivela, E., Wray, V., Pieper, D. H., Wittich, R.-M. (1999). Initial Reactions in the Biodegradation of 1-Chloro-4-Nitrobenzene by a Newly Isolated Bacterium, Strain LW1. Appl. Environ. Microbiol. 65: 1405-1412 [Abstract] [Full Text]  
• Park, H.-S., Lim, S.-J., Chang, Y. K., Livingston, A. G., Kim, H.-S. (1999). Degradation of Chloronitrobenzenes by a Coculture of Pseudomonas putida and a Rhodococcus sp.. Appl. Environ. Microbiol. 65: 1083-1091 [Abstract] [Full Text]  
• Hay, A. G., Focht, D. D. (1998). Cometabolism of 1,1-Dichloro-2,2-Bis(4-Chlorophenyl)Ethylene by Pseudomonas acidovorans M3GY Grown on Biphenyl. Appl. Environ. Microbiol. 64: 2141-2146 [Abstract] [Full Text]  
• Kaschabek, S. R., Kasberg, T., Müller, D., Mars, A. E., Janssen, D. B., Reineke, W. (1998). Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of Pseudomonas putida GJ31. J. Bacteriol. 180: 296-302 [Abstract] [Full Text]