This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, G. C. Articles by Wang, Y. Search for Related Content PubMed PubMed Citation Articles by Wang, G. C. Articles by Wang, Y. Agricola Articles by Wang, G. C. Articles by Wang, Y.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 12 1995, 4269-4273, Vol 61, No. 12
Copyright © 1995, American Society for Microbiology

Rapid differentiation of bacterial species with multiple probes of different lengths in a single slot blot hybridization

GC Wang and Y Wang
Microbial Collection and Screen Laboratory, National University of Singapore, Singapore.

We describe a highly efficient method for dot and slot blot hybridizations with multiple oligonucleotide probes for high throughput identification of organisms and studies of microbial community structures. Several probes with distinct specificities were designed to have the same melting temperature but unique lengths by adding different numbers of nonspecific nucleotides to one end. All of the probes were mixed, labelled with 32P, and hybridized to one piece of membrane on which genes coding for 16S rRNAs from different bacterial species had been immobilized. After hybridization, the bound probes were eluted and resolved on a denaturing polyacrylamide gel and the identities of the genes coding for 16S rRNAs were read from an autoradiograph of the gel. The results from the application of this technique to pure actinomycete cultures are reported here.

This article has been cited by other articles:

• Smalla, K., Wieland, G., Buchner, A., Zock, A., Parzy, J., Kaiser, S., Roskot, N., Heuer, H., Berg, G. (2001). Bulk and Rhizosphere Soil Bacterial Communities Studied by Denaturing Gradient Gel Electrophoresis: Plant-Dependent Enrichment and Seasonal Shifts Revealed. Appl. Environ. Microbiol. 67: 4742-4751 [Abstract] [Full Text]  
• Okhravi, N., Adamson, P., Matheson, M. M., Towler, H. M. A., Lightman, S. (2000). PCR-RFLP-Mediated Detection and Speciation of Bacterial Species Causing Endophthalmitis. IOVS 41: 1438-1447 [Abstract] [Full Text]