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Appl. Environ. Microbiol., Oct 1995, 3756-3758, Vol 61, No. 10
Copyright © 1995, American Society for Microbiology

Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts

EM Powers
U.S. Army Soldier System Command, Natick Research, Development and Engineering Center, Massachusetts 01760-5018, USA.

A simple and rapid (< 60 s) nonstaining technique with 3% potassium hydroxide to determine Gram reactions was tested with 495 food-borne and waterborne bacteria and yeasts. In KOH, suspensions of gram- negative bacteria become viscous and string out. Gram-positive bacteria are not affected. There was 100% correlation between the KOH string test results and gram-positive and gram-negative strains.

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