This Article Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schraft, H. Articles by Griffiths, M. W. Search for Related Content PubMed PubMed Citation Articles by Schraft, H. Articles by Griffiths, M. W. Agricola Articles by Schraft, H. Articles by Griffiths, M. W.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 01 1995, 98-102, Vol 61, No. 1
Copyright © 1995, American Society for Microbiology

Specific oligonucleotide primers for detection of lecithinase-positive Bacillus spp. by PCR [published erratum appears in Appl Environ Microbiol 1995 Jun;61(6):2452]

H Schraft and MW Griffiths
Department of Food Science, University of Guelph, Ontario, Canada.

An assay based on the PCR has been developed to facilitate detection and identification of Bacillus cereus in foods. Three primers for the PCR have been designed within the sequence for cereolysin AB, a cytolytic determinant that encodes lecithin-hydrolyzing and hemolytic activities of B. cereus. With the PCR and hybridization, the specificity of the primers was tested with 39 isolates of the B. cereus group, with 17 other Bacillus spp., and with 21 non-Bacillus strains. Results demonstrate a high specificity of the three oligonucleotides for isolates of the B. cereus group. With a combined PCR-hybridization assay, the detection limit for B. cereus in artificially contaminated milk was 1 CFU/ml of milk.

This article has been cited by other articles:

• Lee, S. J., Park, S.-Y., Lee, J.-J., Yum, D.-Y., Koo, B.-T., Lee, J.-K. (2002). Genes Encoding the N-Acyl Homoserine Lactone-Degrading Enzyme Are Widespread in Many Subspecies of Bacillus thuringiensis. Appl. Environ. Microbiol. 68: 3919-3924 [Abstract] [Full Text]  
• Qi, Y., Patra, G., Liang, X., Williams, L. E., Rose, S., Redkar, R. J., DelVecchio, V. G. (2001). Utilization of the rpoB Gene as a Specific Chromosomal Marker for Real-Time PCR Detection of Bacillus anthracis. Appl. Environ. Microbiol. 67: 3720-3727 [Abstract] [Full Text]  
• Brumlik, M. J., Szymajda, U., Zakowska, D., Liang, X., Redkar, R. J., Patra, G., Del Vecchio, V. G. (2001). Use of Long-Range Repetitive Element Polymorphism-PCR To Differentiate Bacillus anthracis Strains. Appl. Environ. Microbiol. 67: 3021-3028 [Abstract] [Full Text]  
• Daffonchio, D., Cherif, A., Borin, S. (2000). Homoduplex and Heteroduplex Polymorphisms of the Amplified Ribosomal 16S-23S Internal Transcribed Spacers Describe Genetic Relationships in the "Bacillus cereus Group". Appl. Environ. Microbiol. 66: 5460-5468 [Abstract] [Full Text]  
• Kim, Y.-R., Czajka, J., Batt, C. A. (2000). Development of a Fluorogenic Probe-Based PCR Assay for Detection of Bacillus cereus in Nonfat Dry Milk. Appl. Environ. Microbiol. 66: 1453-1459 [Abstract] [Full Text]  
• Daffonchio, D., Borin, S., Frova, G., Gallo, R., Mori, E., Fani, R., Sorlini, C. (1999). A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis. Appl. Environ. Microbiol. 65: 1298-1303 [Abstract] [Full Text]