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Appl Environ Microbiol. 1993 June; 59(6): 1943-1946

Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method.

First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.

ABSTRACT

The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.

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