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Appl Environ Microbiol. 1993 April; 59(4): 953-959
Copyright © 1993, American Society for Microbiology. All Rights Reserved.

A New Way of Producing Isomalto-Oligosaccharide Syrup by Using the Transglycosylation Reaction of Neopullulanase

¹ Biochemical Research Laboratories, Ezaki Glico Co., Ltd., Nishiyodogawa-ku, Osaka 555, Japan
² Nagase Biochemicals Co., Ltd., Nagatano-cho, Fukuchiyama, Kyoto 620, Japan
³ Department of Biotechnology, Faculty of Engineering, Osaka University, Yamadaoka, Suita, Osaka 565, Japan

ABSTRACT

A new way of producing isomalto-oligosaccharide syrup from starch was developed. Isomalto-oligosaccharides contain one or more -(16)-glucosidic linkages with or without -(14)-glucosidic linkages. The isomalto-oligosaccharide syrups are receiving increased attention as food additives because it is thought that they help prevent dental caries and improve human intestinal microflora, acting as a growth factor for bifidobacteria. The new system for production of isomalto-oligosaccharide syrup is based on the strong -(16)-transglycosylation reaction of neopullulanase. Bacillus subtilis saccharifying -amylase was simultaneously used with neopullulanase to improve the yield of isomalto-oligosaccharides. The yield of isomalto-oligosaccharides was increased to more than 60%, compared with a yield of 45.0% obtained by the conventional system. To reduce the costs, the use of immobilized neopullulanase was investigated. Almost the same yield of isomalto-oligosaccharides was obtained by using immobilized neopullulanase.

^* Corresponding author.

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