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Appl Environ Microbiol. 1993 March; 59(3): 915-918

Direct extraction and purification of rRNA for ecological studies.

Department of Microbiology, University of Georgia, Athens 30602-2605.

ABSTRACT

Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.

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