This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Koch, W H Articles by Cebula, T A Search for Related Content PubMed PubMed Citation Articles by Koch, W H Articles by Cebula, T A Agricola Articles by Koch, W H Articles by Cebula, T A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 February; 59(2): 556-560

Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.

Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

ABSTRACT

The polymerase chain reaction was used to selectively amplify sequences within the cholera toxin operon from Vibrio cholerae O1. Oysters, crabmeat, shrimp, and lettuce were seeded with V. cholerae and then homogenized or washed with alkaline peptone water, followed by short-term (6- to 8-h) enrichment. A detection limit of as few as 1 V. cholerae CFU per 10 g of food was obtained with amplification reactions from crude bacterial lysates. The method is extremely rapid and obviates the need for DNA isolation from a variety of complex food matrices.

This article has been cited by other articles:

• Morin, N. J., Gong, Z., Li, X.-F. (2004). Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi. Clin. Chem. 50: 2037-2044 [Abstract] [Full Text]  
• Hong, Y., Berrang, M. E., Liu, T., Hofacre, C. L., Sanchez, S., Wang, L., Maurer, J. J. (2003). Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay. Appl. Environ. Microbiol. 69: 3492-3499 [Abstract] [Full Text]  
• Goosen, C., Theron, J., Ntsala, M., Maree, F. F., Olckers, A., Botha, S. J., Lastovica, A. J., van der Merwe, S. W. (2002). Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque. J. Clin. Microbiol. 40: 205-209 [Abstract] [Full Text]  
• Lyon, W. J. (2001). TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater. Appl. Environ. Microbiol. 67: 4685-4693 [Abstract] [Full Text]