This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kato, J Articles by Ohtake, H Search for Related Content PubMed PubMed Citation Articles by Kato, J Articles by Ohtake, H Agricola Articles by Kato, J Articles by Ohtake, H

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 November; 59(11): 3744-3749

Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater.

Department of Fermentation Technology, Hiroshima University, Japan.

ABSTRACT

The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kinase (ppk), acetate kinase (ackA), and the phosphate-inducible transport system (pstS, pstC, pstA, and pstB). Acetate kinase was employed as an ATP regeneration system for polyphosphate synthesis. Recombinant strains, which contained either pBC29 (carrying ppk) or pEP02.2 (pst operon), removed approximately two- and threefold, respectively, more Pi from minimal medium than did the control strain. The highest rates of Pii removal were obtained by strain MV1184 containing pEP03 (ppk and ackA). However, unlike the control strain, MV1184 (pEP03) released Pi to the medium after growth had stopped. Drastic changes in growth and Pi uptake were observed when pBC29 (ppk) and pEP02.2 (pst operon) were introduced simultaneously into MV1184. Even though growth of this recombinant was severely limited in minimal medium, the recombinant could remove approximately threefold more Pi than the control strain. Consequently, the phosphorus content of this recombinant reached a maximum of approximately 16% on a dry weight basis (49% as phosphate).

This article has been cited by other articles:

• Morohoshi, T., Maruo, T., Shirai, Y., Kato, J., Ikeda, T., Takiguchi, N., Ohtake, H., Kuroda, A. (2002). Accumulation of Inorganic Polyphosphate in phoU Mutants of Escherichia coli and Synechocystis sp. Strain PCC6803. Appl. Environ. Microbiol. 68: 4107-4110 [Abstract] [Full Text]  
• Imazu, K., Tanaka, S., Kuroda, A., Anbe, Y., Kato, J., Ohtake, H. (1998). Enhanced Utilization of Phosphonate and Phosphite by Klebsiella aerogenes. Appl. Environ. Microbiol. 64: 3754-3758 [Abstract] [Full Text]