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Appl Environ Microbiol. 1992 September; 58(9): 3012-3019
Copyright © 1992, American Society for Microbiology. All Rights Reserved.

Persistence of Free Plasmid DNA in Soil Monitored by Various Methods, Including a Transformation Assay

Gerd Romanowski, Michael G. Lorenz*, Gary Sayler and Wilfried Wackernagel

1 Genetik, Fachbereich Biologie, Universität Oldenburg, Postfach 2503, W-2900 Oldenburg, Germany, and Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 379322

ABSTRACT

The persistence and stability of free plasmid pUC8-ISP DNA introduced into 10-g samples of various soils and kept at 23°C were monitored over a period of 60 days. The soils were sampled at a plant science farm and included a loamy sand soil (no. 1), a clay soil (no. 2), and a silty clay soil (no. 3). Four different methods allowed monitoring of (i) the production of acid-soluble radioactive material from [3H]thymidine-labeled plasmid DNA, (ii) the decrease of hybridizing nucleotide sequences in slot blot analysis, (iii) the loss of plasmid integrity measured by Southern hybridization, and (iv) the decay of the biological activity as determined by transformation of Ca2+-treated Escherichia coli cells with the DNA extracted from soil. Acid-soluble material was not produced within the first 24 h but then increased to 45% (soil no. 1), 27% (soil no. 2), and 77% (soil no. 3) until the end of incubation. A quite parallel loss of material giving a slot blot hybridization signal was observed. Southern hybridization indicated that after 1 h in the soils, plasmid DNA was mostly in the form of circular and full-length linear molecules but that, depending on the soil type, after 2 to 5 days full-length plasmid molecules were hardly detectable. The transforming activity of plasmid DNA reextracted from the soils followed inactivation curves over 2 to 4 orders of magnitude and dropped below the detection limit after 10 days. The inactivation was slower in soil no. 2 (28.2-h half-life time of the transforming activity of a plasmid molecule) than in soils no. 3 (15.1 h) and no. 1 (9.1 h). The studies provide data on the persistence of free DNA molecules in natural bacterial soil habitats. The data suggest that plasmid DNA may persist long enough to be available for uptake by competent recipient cells in situ.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1992 September; 58(9): 3012-3019
Copyright © 1992, American Society for Microbiology. All Rights Reserved.



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