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Appl Environ Microbiol. 1992 September; 58(9): 2983-2992
Copyright © 1992, American Society for Microbiology. All Rights Reserved.

Evaluation of Methyl Fluoride and Dimethyl Ether as Inhibitors of Aerobic Methane Oxidation

Ronald S. Oremland* and Charles W. Culbertson

U.S. Geological Survey, ms 465, 345 Middlefield Road, Menlo Park, California 94025

ABSTRACT

Methyl fluoride (MF) and dimethyl ether (DME) were effective inhibitors of aerobic methanotrophy in a variety of soils. MF and DME blocked consumption of CH4 as well as the oxidation of 14CH4 to 14CO2, but neither MF nor DME affected the oxidation of [14C]methanol or [14C]formate to 14CO2. Cooxidation of ethane and propane by methane-oxidizing soils was also inhibited by MF. Nitrification (ammonia oxidation) in soils was inhibited by both MF and DME. Production of N2O via nitrification was inhibited by MF; however, MF did not affect N2O production associated with denitrification. Methanogenesis was partially inhibited by MF but not by DME. Methane oxidation was ~100-fold more sensitive to MF than was methanogenesis, indicating that an optimum concentration could be employed to selectively block methanotrophy. MF inhibited methane oxidation by cell suspensions of Methylococcus capsulatus; however, DME was a much less effective inhibitor.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1992 September; 58(9): 2983-2992
Copyright © 1992, American Society for Microbiology. All Rights Reserved.



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