This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Page, W J Articles by Rudy, B Search for Related Content PubMed PubMed Citation Articles by Page, W J Articles by Rudy, B Agricola Articles by Page, W J Articles by Rudy, B

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 September; 58(9): 2866-2873

Formation of poly(hydroxybutyrate-co-hydroxyvalerate) by Azotobacter vinelandii UWD.

Department of Microbiology, University of Alberta, Edmonton, Canada.

ABSTRACT

Azotobacter vinelandii UWD formed polyhydroxyalkanoate (PHA) copolymers containing beta-hydroxybutyrate and beta-hydroxyvalerate (HV) when grown in a medium containing glucose as the primary C source and valerate (pentanoate) as a precursor. Copolymer was not formed when propionate was added to the glucose medium but was formed when heptanoate, nonanoate, or trans-2-pentenoate was present. Optimal levels of HV were formed when valerate was added at the time of maximum PHA synthesis, although HV incorporation was not dependent on glucose catabolism. HV content in the polymer was increased from 17 to 24 mol% by adding 10 to 40 mM valerate to glucose medium, but HV insertion into the polymer occurred at a fixed rate. Similarly, the addition of valerate to a fed-batch culture of strain UWD in beet molasses in a fermentor produced 19 to 22 g of polymer per liter, containing 8.5 to 23 mol% HV after 38 to 40 h. The synthesis of HV in these cultures also occurred at a fixed rate (2.3 to 2.8 mol% h-1), while the maximum PHA production rate was 1.1 g liter-1 h-1. During synthesis of copolymer in batch or fed-batch culture, the yield from conversion of glucose into PHA (YP/S) remained at maximum theoretical efficiency (greater than or equal to 0.33 g of PHA per g of glucose consumed). Up to 45 mol% C source, but the PHA produced amounted to less than 1 g/liter. The combination of 30 mM valerate as a sole C source and 0.5 mM 4-pentenoate increased the HV content in the polymer to 52 mol%.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

• Pettinari, M. J., Vazquez, G. J., Silberschmidt, D., Rehm, B., Steinbuchel, A., Mendez, B. S. (2001). Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8. Appl. Environ. Microbiol. 67: 5331-5334 [Abstract] [Full Text]  
• Page, W. J., Tindale, A., Chandra, M., Kwon, E. (2001). Alginate formation in Azotobacter vinelandii UWD during stationary phase and the turnover of poly-{beta}-hydroxybutyrate. Microbiology 147: 483-490 [Abstract] [Full Text]  
• Choi, J.-i., Lee, S. Y. (1999). High-Level Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of Recombinant Escherichia coli. Appl. Environ. Microbiol. 65: 4363-4368 [Abstract] [Full Text]  
• Madison, L. L., Huisman, G. W. (1999). Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic. Microbiol. Mol. Biol. Rev. 63: 21-53 [Abstract] [Full Text]