AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Kuppeveld, F J
Right arrow Articles by Melchers, W J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Kuppeveld, F J
Right arrow Articles by Melchers, W J
Agricola
Right arrow Articles by van Kuppeveld, F J
Right arrow Articles by Melchers, W J

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 August; 58(8): 2606-2615

Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.

F J van Kuppeveld, J T van der Logt, A F Angulo, M J van Zoest, W G Quint, H G Niesters, J M Galama and W J Melchers

Department of Medical Microbiology, University of Nijmegen, The Netherlands.

ABSTRACT

Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the identification of variable regions with both genus- and species-specific sequences. Species-specific sequences of Mycoplasma collis were elucidated by asymmetric amplification and dideoxynucleotide sequencing of variable regions, using primers complementary to conserved regions of 16S rRNA. Primers selected for Mycoplasma pneumoniae, M. hominis, M. fermentans, Ureaplasma urealyticum, M. pulmonis, M. arthritidis, M. neurolyticum, M. muris, and M. collis proved to be species specific in the polymerase chain reaction. The genus-specific primers reacted with all mycoplasmal species investigated as well as with members of the genera Ureaplasma, Spiroplasma, and Acholeplasma. No cross-reaction was observed with members of the closely related genera Streptococcus, Lactobacillus, Bacillus, and Clostridium or with any other microorganism tested. On the basis of the high copy number of rRNA, a highly sensitive polymerase chain reaction assay was developed in which the nucleic acid content equivalent to a single organism could be detected.

Appl Environ Microbiol. 1992 August; 58(8): 2606-2615




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1992 by the American Society for Microbiology. All rights reserved.