This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Higson, F K Articles by Focht, D D Search for Related Content PubMed PubMed Citation Articles by Higson, F K Articles by Focht, D D Agricola Articles by Higson, F K Articles by Focht, D D

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 August; 58(8): 2501-2504

Utilization of 3-chloro-2-methylbenzoic acid by Pseudomonas cepacia MB2 through the meta fission pathway.

Department of Soil and Environmental Sciences, University of California, Riverside 92521.

ABSTRACT

Pseudomonas cepacia MB2 grew on 3-chloro-2-methylbenzoate as a sole carbon source by metabolism through the meta fission pathway with the subsequent liberation of chloride. meta pyrocatechase activity in cell extracts was induced strongly by 3-chloro-2-methylbenzoate, but not by nongrowth analogs 4- or 5-chloro-2-methylbenzoate. Although rapid turnover of metabolites precluded direct identification, a mutant strain MB2-G5 lacking meta pyrocatechase activity produced 4-chloro-3-methylcatechol when incubated with 3-chloro-2-methylbenzoate. The catecholic product, confirmed by nuclear magnetic resonance and mass spectral analyses, produced a transient meta fission product (lambda max = 391 nm) from cell extracts of the wild-type MB2 strain. Further confirmation of meta pyrocatechase activity was noted by conversion of 4-chlorocatechol to 2-hydroxy-5-chloromuconic semialdehyde, which was not further metabolized. In contrast to 3-chlorocatechol, which was not metabolized and is known to generate suicidal products, 4-chlorocatechols do not generate acyl halides. Thus, further metabolism of the ring fission products is governed in strain MB2 by their suitability as substrates for the hydrolase.

This article has been cited by other articles:

• Mars, A. E., Kingma, J., Kaschabek, S. R., Reineke, W., Janssen, D. B. (1999). Conversion of 3-Chlorocatechol by Various Catechol 2,3-Dioxygenases and Sequence Analysis of the Chlorocatechol Dioxygenase Region of Pseudomonas putida GJ31. J. Bacteriol. 181: 1309-1318 [Abstract] [Full Text]  
• Riegert, U., Heiss, G., Fischer, P., Stolz, A. (1998). Distal Cleavage of 3-Chlorocatechol by an Extradiol Dioxygenase to 3-Chloro-2-Hydroxymuconic Semialdehyde. J. Bacteriol. 180: 2849-2853 [Abstract] [Full Text]  
• Kaschabek, S. R., Kasberg, T., Müller, D., Mars, A. E., Janssen, D. B., Reineke, W. (1998). Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of Pseudomonas putida GJ31. J. Bacteriol. 180: 296-302 [Abstract] [Full Text]