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Appl Environ Microbiol. 1992 April; 58(4): 1379-1381

Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase.

J Gaskell, A Vanden Wymelenberg, P Stewart and D Cullen

Institute for Microbial and Biochemical Technology, USDA Forest Service, Madison, Wisconsin 53705.

ABSTRACT

A method to identify and differentiate allelic variants of the gene encoding lignin peroxidase isozyme H8 is presented. The strategy involves amplifying a variable region of the gene's carboxy terminus by use of the polymerase chain reaction and then probing with allele-specific oligonucleotides.

Appl Environ Microbiol. 1992 April; 58(4): 1379-1381




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