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Appl Environ Microbiol. 1992 February; 58(2): 614-623

Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.

Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.

ABSTRACT

The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.

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