This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van Belkum, M J Articles by Venema, G Search for Related Content PubMed PubMed Citation Articles by van Belkum, M J Articles by Venema, G Agricola Articles by van Belkum, M J Articles by Venema, G

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 February; 58(2): 572-577

Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.

Department of Genetics, University of Groningen, Haren, The Netherlands.

ABSTRACT

On the bacteriocin plasmid p9B4-6 of Lactococcus lactis subsp. cremoris 9B4, a third bacteriocin determinant was identified. The genes encoding bacteriocin production and immunity resided on a 1.2-kb CelII-ScaI fragment and were located adjacent to one of two previously identified bacteriocin operons (M. J. van Belkum, B. J. Hayema, R. E. Jeeninga, J. Kok, and G. Venema, Appl. Environ. Microbiol. 57:492-498, 1991). The fragment was sequenced and analyzed by deletion and mutation analyses. The bacteriocin determinant consisted of two genes which were transcribed as an operon. The first gene (lcnB), containing 68 codons, was involved in bacteriocin activity. The second gene (lciB) contained 91 codons and was responsible for immunity. The specificity of this novel bacteriocin, designated lactococcin B, was different from that of the other two bacteriocins specified by p9B4-6. Part of the nucleotide sequence of the lactococcin B operon was similar to a nucleotide sequence also found in the two other bacteriocin operons of p9B4-6. This conserved region encompassed a nucleotide sequence upstream of the bacteriocin gene and the 5' part of the gene. When the lactococcin B operon was expressed in Escherichia coli by using a T7 RNA polymerase-specific promoter, antagonistic activity could be detected.

This article has been cited by other articles:

• Zendo, T., Koga, S., Shigeri, Y., Nakayama, J., Sonomoto, K. (2006). Lactococcin Q, a Novel Two-Peptide Bacteriocin Produced by Lactococcus lactis QU 4.. Appl. Environ. Microbiol. 72: 3383-3389 [Abstract] [Full Text]  
• Kalmokoff, M. L., Banerjee, S. K., Cyr, T., Hefford, M. A., Gleeson, T. (2001). Identification of a New Plasmid-Encoded sec-Dependent Bacteriocin Produced by Listeria innocua 743. Appl. Environ. Microbiol. 67: 4041-4047 [Abstract] [Full Text]  
• Kalmokoff, M. L., Lu, D., Whitford, M. F., Teather, R. M. (1999). Evidence for Production of a New Lantibiotic (Butyrivibriocin OR79A) by the Ruminal Anaerobe Butyrivibrio fibrisolvens OR79: Characterization of the Structural Gene Encoding Butyrivibriocin OR79A. Appl. Environ. Microbiol. 65: 2128-2135 [Abstract] [Full Text]  
• O'Keeffe, T., Hill, C., Ross, R. P. (1999). Characterization and Heterologous Expression of the Genes Encoding Enterocin A Production, Immunity, and Regulation in Enterococcus faecium DPC1146. Appl. Environ. Microbiol. 65: 1506-1515 [Abstract] [Full Text]  
• Fleury, Y., Dayem, M. A., Montagne, J. J., Chaboisseau, E., Caer, J. P. L., Nicolas, P., Delfour, A. (1996). Covalent Structure, Synthesis, and Structure-Function Studies of Mesentericin Y 10537, a Defensive Peptide from Gram-positive Bacteria Leuconostoc mesenteroides. J. Biol. Chem. 271: 14421-14429 [Abstract] [Full Text]