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Appl Environ Microbiol. 1992 December; 58(12): 4051-4054

Subculturing of a polychlorinated biphenyl-dechlorinating anaerobic enrichment on solid media.

H D May, A W Boyle, W A Price 2nd and C K Blake

Celgene Corp., Warren, New Jersey 07059.

ABSTRACT

An anaerobic culture capable of dechlorinating polychlorinated biphenyls was subcultured under strict anaerobic conditions on solid media containing sterilized river sediment. The dechlorination activity was transferred as a bacterial colony on a solid medium three times. After two transfers on solid medium, the culture was no longer methanogenic but still dechlorinated a mixture of tri- and tetrachlorobiphenyls. This demonstrates that anaerobic bacteria are responsible for the polychlorinated biphenyl dechlorination and can be grown without polychlorinated biphenyl on solid media.

Appl Environ Microbiol. 1992 December; 58(12): 4051-4054




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