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Appl Environ Microbiol. 1992 December; 58(12): 3864-3867

The beta-mannanase from "Caldocellum saccharolyticum" is part of a multidomain enzyme.

Department of Cellular & Molecular Biology, University of Auckland, New Zealand.

ABSTRACT

The complete sequence of a beta-mannanase gene from an anaerobic extreme thermophile was determined, and it shows that the expressed protein consists of two catalytic domains and two binding domains separated by spacer regions rich in proline and threonine residues. The amino-terminal catalytic domain has beta-mannanase activity, and the carboxy-terminal domain acts as an endoglucanase. Neither domain shows homology with any other cellulase or hemicellulase sequence at the nucleic acid or protein level.

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