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Appl Environ Microbiol. 1992 November; 58(11): 3538-3541

Application of gas chromatography-mass spectrometry for rapid detection of Mycobacterium xenopi in drinking water.

Department of Medical Microbiology, University of Lund, Sweden.

ABSTRACT

Gas chromatography-mass spectrometry (GC-MS) was used to detect 2-docosanol, a secondary alcohol characteristic of Mycobacterium xenopi, in 7 of 10 analyzed drinking water samples culture positive for that species. GC-MS was also used to detect tuberculostearic acid. Both of these chemical markers were analyzed as halogenated derivatives in the negative-ion-chemical-ionization mode. The numbers of CFU of M. xenopi were lowest in the three GC-MS-negative samples. The described GC-MS method is useful for the rapid detection of M. xenopi in drinking water.