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Appl Environ Microbiol. 1992 November; 58(11): 3443-3447

Discrimination of Listeria monocytogenes from other Listeria species by ligase chain reaction.

Department of Food Science, Cornell University, Ithaca, New York 14853.

ABSTRACT

A ligase chain reaction assay based on a single-base-pair difference in the V9 region of the 16S rRNA gene (16S rDNA) was developed to distinguish between Listeria monocytogenes and other Listeria species. For this purpose, two pairs of primers were designed, with one primer of each pair being radioactively labeled. The ligated product was separated from the primers by denaturing polyacrylamide gel electrophoresis and then detected by autoradiography. To achieve a higher sensitivity, the 16S rDNA was initially amplified by polymerase chain reaction prior to the ligase chain reaction. The ligase chain reaction was tested on 19 different Listeria species and strains and proved to be a highly specific diagnostic method for the detection of L. monocytogenes.

This article has been cited by other articles:

• Norton, D.-M., Batt, C. A. (1999). Detection of Viable Listeria monocytogenes with a 5' Nuclease PCR Assay. Appl. Environ. Microbiol. 65: 2122-2127 [Abstract] [Full Text]  
• Wiedmann, M, Wilson, W J, Czajka, J, Luo, J, Barany, F, Batt, C A (1994). Ligase chain reaction (LCR)--overview and applications.. Genome Res 3: S51-S64