This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Goodnough, M C Articles by Johnson, E A Search for Related Content PubMed PubMed Citation Articles by Goodnough, M C Articles by Johnson, E A Agricola Articles by Goodnough, M C Articles by Johnson, E A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 October; 58(10): 3426-3428

Stabilization of botulinum toxin type A during lyophilization.

Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706.

ABSTRACT

Botulinum toxin for medical use is diluted to very low concentrations (nanograms per milliliter); when it is preserved by lyophilization, considerable loss of activity can occur. In the present study, conditions that gave > 90% recovery of the toxicity after lyophilization of solutions containing 20 to 1,000 mouse 50% lethal doses per ml were found. Toxicity was recovered upon drying 0.1 ml of toxin solution when the pH was maintained below 7 and bovine or human serum albumins were used as stabilizers. Various other substances tested with albumin, including glucose, sucrose, trehalose, mannitol, glycine, and cellibiose, did not increase recovery on drying.

This article has been cited by other articles:

• Arimitsu, H., Inoue, K., Sakaguchi, Y., Lee, J., Fujinaga, Y., Watanabe, T., Ohyama, T., Hirst, R., Oguma, K. (2003). Purification of Fully Activated Clostridium botulinum Serotype B Toxin for Treatment of Patients with Dystonia. Infect. Immun. 71: 1599-1603 [Abstract] [Full Text]