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Appl Environ Microbiol. 1992 October; 58(10): 3413-3416

Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction.

Department of Genetics and Microbiology, Donnan Laboratories, University of Liverpool, United Kingdom.

ABSTRACT

Specific DNA sequences from native bacterial populations present in soil, sediment, and sand samples were amplified by using the polymerase chain reaction with primers for either "universal" eubacterial 16S rRNA genes or mercury resistance (mer) genes. With standard amplification conditions, 1.5-kb rDNA fragments from all 12 samples examined and from as little as 5 micrograms of soil were reproducibly amplified. A 1-kb mer fragment from one soil sample was also amplified. The identity of these amplified fragments was confirmed by DNA-DNA hybridization.

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