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Appl Environ Microbiol. 1992 October; 58(10): 3367-3373
Copyright © 1992, American Society for Microbiology. All Rights Reserved.

Comparison of Identification Systems for Classification of Bacteria Isolated from Water and Endolithic Habitats within the Deep Subsurface

P. S. Amy*, D. L. Haldeman, D. Ringelberg, D. H. Hall and C. Russell

1 Department of Biological Sciences, University of Nevada, Las Vegas, Las Vegas, Nevada 89154; Desert Research Institute, Las Vegas, Nevada 891203; and Institute for Applied Microbiology, University of Tennessee, Knoxville, Tennessee 379322

ABSTRACT

One water and three rock samples were taken from a mined tunnel system, U12n, in Rainier Mesa at the Nevada Test Site. Endolithic microorganisms were cultured from ashfall tuff, which was crushed and made into slurries with a formulation of artificial pore water, on R2A agar plates. Microbial counts ranged from 102 to 104 viable cells per g (dry weight) of rock sampled. The cultured water sample yielded 102 viable cells per ml. Many of the isolates were very small (<1 µm) when viewed in the rock matrix and remained small even when cultured. Most were gram-negative rods. Individual isolates were profiled by API-NFT strip number, antibiotic and metal resistance patterns, and colony and cellular morphologies. Three identification systems, API-NFT strips, BIOLOG, and MIDI, were compared. Each system identified only a small percentage of the total isolates, and in only seven cases were the isolates identified the same way by more than one system. The same genus was identified in three of these cases, but different species were indicated. The genus Pseudomonas was the most commonly identified. The isolate profiles and the three identification systems demonstrated that water isolates were considerably different from endolithic isolates.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1992 October; 58(10): 3367-3373
Copyright © 1992, American Society for Microbiology. All Rights Reserved.



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Copyright © 1992 by the American Society for Microbiology. All rights reserved.