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Appl Environ Microbiol. 1992 October; 58(10): 3217-3224
Copyright © 1992, American Society for Microbiology. All Rights Reserved.

Solubilization and Mineralization of Lignin by White Rot Fungi

C. David Boyle^,*, Bradley R. Kropp and Ian D. Reid

¹ Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Université Laval, Sainte-Foy, Québec, Canada G1K 7P4, and Biotechnology Research Council, National Research Council of Canada, Montreal, Québec, Canada H4P 2R2²

ABSTRACT

The white rot fungi Lentinula edodes, Phanerochaete chrysosporium, Pleurotus sajor-caju, Flammulina velutipes, and Schizophyllum commune were grown in liquid media containing ¹⁴C-lignin-labelled wood, and the formation of water-soluble ¹⁴C-labelled products and ¹⁴CO₂, the growth of the fungi, and the activities of extracellular lignin peroxidase, manganese peroxidase, and laccase were measured. Conditions that affect the rate of lignin degradation were imposed, and both long-term (0- to 16-day) and short-term (0- to 72-h) effects on the production of the two types of product and on the activities of the enzymes were monitored. The production of ¹⁴CO₂-labelled products from the aqueous ones was also investigated. The short-term studies showed that the different conditions had different effects on the production of the two products and on the activities of the enzymes. Nitrogen sources inhibited the production of both products by all species when differences in growth could be discounted. Medium pH and manganese affected lignin degradation by the different species differently. With P. chrysosporium, the results were consistent, with lignin peroxidase playing a role in lignin solubilization and manganese peroxidase being important in subsequent CO₂ production.

^* Corresponding author.

Present address: The Research and Productivity Council, 921 College Hill Rd., Fredericton, New Brunswick, Canada E3B 6Z9.

Present address: Biology UMC 5305, Utah State University, Logan, UT 84322.

Present address: Pulp and Paper Research Institute of Canada, Pointe-Claire, Québec, Canada H9R 3J9.

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