This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vullo, D L Articles by Siñeriz, F Search for Related Content PubMed PubMed Citation Articles by Vullo, D L Articles by Siñeriz, F Agricola Articles by Vullo, D L Articles by Siñeriz, F

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 August; 57(8): 2392-2394

Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby).

Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales (UBA), Ciudad Universitaria, Buenos Aires, Argentina.

ABSTRACT

Bacillus subtilis 430A, isolated from the Vernonia herbacea (Vell Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for inulin (Km, 8 mM) than for sucrose (56 mM) and a total invertase/total inulase ratio of 0.62. In addition, it is stable at an optimal temperature of 45 to 50 degrees C for at least 7 h and is inhibited by the end product, fructose, at 14 mM.

This article has been cited by other articles:

• Kinsinger, R. F., Shirk, M. C., Fall, R. (2003). Rapid Surface Motility in Bacillus subtilis Is Dependent on Extracellular Surfactin and Potassium Ion. J. Bacteriol. 185: 5627-5631 [Abstract] [Full Text]