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Appl Environ Microbiol. 1991 July; 57(7): 2097-2100

Rapid and sensitive assay for the phytotoxin rhizobitoxine.

X Ruan and N K Peters

Department of Agronomy, Ohio State University, Columbus 43210-1002.

ABSTRACT

Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM.

Appl Environ Microbiol. 1991 July; 57(7): 2097-2100




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