This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Engelking, H. M. Articles by Leong, J.-A. C. Search for Related Content PubMed PubMed Citation Articles by Engelking, H. M. Articles by Leong, J.-A. C. Agricola Articles by Engelking, H. M. Articles by Leong, J.-A. C.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 May; 57(5): 1372-1378
Copyright © 1991, American Society for Microbiology. All Rights Reserved.

Comparison of Representative Strains of Infectious Hematopoietic Necrosis Virus by Serological Neutralization and Cross-Protection Assays

¹ Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-3804, and Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, California 90024-1747²

ABSTRACT

Infectious hematopoietic necrosis virus (IHNV) is a pathogen of young salmon and trout. Viral epizootics among these fish in private and public rearing facilities have been a problem in the northwestern United States from California to Alaska, and an IHNV vaccine has been sought by the aquaculture experts. Since an IHNV vaccine must be designed to immunize against all viral serotypes, an analysis of IHNV serotypes was made. A large number of viruses from widely separated geographic locations and different fish species had already been placed in one of five electropherotypes by the migration of the virion proteins in sodium dodecyl sulfate-polyacrylamide gels. Also, there was evidence that some of these virus isolates had differences in virulence for chinook salmon, rainbow trout, or kokanee salmon. Previous serological studies with polyclonal rabbit antisera and three IHNV isolates indicated that there was only one serotype (B. B. McCain, J. L. Fryer, and K. S. Pilcher, Proc. Soc. Exp. Biol. Med. 137:1042-1046, 1971). A substantial number of new IHNV isolations have been made since that study, and thus a more extensive comparison was made of 10 different IHNV isolates representing the five electropherotypes. This report shows that the glycoprotein from a single isolate of IHNV can induce a protective immune response in vivo to the five IHNV electropherotypes. Plaque reduction neutralization assays indicated that there was only one serotype. Thus, despite the differences observed in the migration of the structural proteins for IHNV isolated from separate geographic locations and different fish species, only one neutralizing virus type was identified.

^* Corresponding author.

Oregon Agricultural Experiment Station Technical Paper no. 9495.

This article has been cited by other articles:

• Liu, X., Collodi, P. (2002). Novel Form of Fibronectin from Zebrafish Mediates Infectious Hematopoietic Necrosis Virus Infection. J. Virol. 76: 492-498 [Abstract] [Full Text]