This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kleman, G L Articles by Strohl, W R Search for Related Content PubMed PubMed Citation Articles by Kleman, G L Articles by Strohl, W R Agricola Articles by Kleman, G L Articles by Strohl, W R

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 April; 57(4): 910-917

A predictive and feedback control algorithm maintains a constant glucose concentration in fed-batch fermentations.

Department of Microbiology, Ohio State University, Columbus 43210.

ABSTRACT

A combined predictive and feedback control algorithm based on measurements of the concentration of glucose on-line has been developed to control fed-batch fermentations of Escherichia coli. The predictive control algorithm was based on the on-line calculation of glucose demand by the culture and plotting a linear regression to the next datum point to obtain a predicted glucose demand. This provided a predictive "coarse" control for the glucose-based nutrient feed. A direct feedback control using a proportional controller, based on glucose measurements every 2 min, fine-tuned the feed rate. These combined control schemes were used to maintain glucose concentrations in fed-batch fermentations as tight as 0.49 +/- 0.04 g/liter during growth of E. coli to high cell densities.

This article has been cited by other articles:

• Winkler, K., Kramer, A., Kuttner, G., Seifert, M., Scholz, C., Wessner, H., Schneider-Mergener, J., Hohne, W. (2000). Changing the Antigen Binding Specificity by Single Point Mutations of an Anti-p24 (HIV-1) Antibody. J. Immunol. 165: 4505-4514 [Abstract] [Full Text]