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Appl Environ Microbiol. 1991 March; 57(3): 879-881
Copyright © 1991, American Society for Microbiology. All Rights Reserved.

Analysis of Free Amino Acids in Microbially Colonized Sandstone by Precolumn Phenyl Isothiocyanate Derivatization and High-Performance Liquid Chromatography

Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, D-2300 Kiel, Federal Republic of Germany

ABSTRACT

A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.

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