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Appl Environ Microbiol. 1991 March; 57(3): 843-846
Copyright © 1991, American Society for Microbiology. All Rights Reserved.

Portable Device for Preparation and Delivery of Gas Mixtures {dagger}

I. J. Misaghi* and L. J. Stowell

1 Department of Plant Pathology, University of Arizona, Tucson, Arizona 85721, and PACE Consulting, San Diego, California 921092

ABSTRACT

A simple, portable device for the preparation and delivery of gas mixtures has been designed and constructed. The basic feature of the device is the use of gas flow controllers to maintain stable flow rates over a wide range of downstream pressures, instead of the capillary tubes and water-filled barostats commonly used in gas-mixing devices. Elimination of the barostat avoids problems such as water leakage, the loss of gases through the barostat, and changes in gas pressure due to evaporative loss of water from the barostat. The absence of a barostat also provides a closed system, allowing the use of the device for mixing and delivering of toxic gases. The prototype of the device has been used to prepare mixtures of different gases for more than 1 year and has been found to operate consistently and reproducibly. The actual concentrations of O2, CO2, and N2 in gas mixtures (determined by gas chromatography) immediately after mixing were between 2.2 and 6.6% of the desired values in four performance tests. Fluctuations in concentration of gases in mixtures after 9 days of continuous gas delivery was less than 2% in four performance tests.

FOOTNOTES

* Corresponding author.

{dagger} This paper is dedicated to the memory of Pat Pascal.

Appl Environ Microbiol. 1991 March; 57(3): 843-846
Copyright © 1991, American Society for Microbiology. All Rights Reserved.



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Copyright © 1991 by the American Society for Microbiology. All rights reserved.