AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deneer, H G
Right arrow Articles by Boychuk, I
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deneer, H G
Right arrow Articles by Boychuk, I
Agricola
Right arrow Articles by Deneer, H G
Right arrow Articles by Boychuk, I

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 February; 57(2): 606-609

Species-specific detection of Listeria monocytogenes by DNA amplification.

H G Deneer and I Boychuk

Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.

ABSTRACT

The polymerase chain reaction was used to detect and specifically identify Listeria monocytogenes. A 174-bp region of the listeriolysin O gene was shown to be specifically amplified in L. monocytogenes but not in other species of Listeria or in a number of other gram-positive and gram-negative organisms. Less than 50 organisms could routinely be detected by a procedure involving two rounds of 35 amplification cycles each and without the need for subsequent hybridization with labeled probes.

Appl Environ Microbiol. 1991 February; 57(2): 606-609




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1991 by the American Society for Microbiology. All rights reserved.