This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nesbakken, T Articles by Hornes, E Search for Related Content PubMed PubMed Citation Articles by Nesbakken, T Articles by Hornes, E Agricola Articles by Nesbakken, T Articles by Hornes, E

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 February; 57(2): 389-394

Comparative study of a DNA hybridization method and two isolation procedures for detection of Yersinia enterocolitica O:3 in naturally contaminated pork products.

Norwegian Meat Research Laboratory, Oslo.

ABSTRACT

We compared a DNA-DNA hybridization assay, using a synthetically produced oligonucleotide probe, and two conventional isolation procedures (methods A and B) with regard to their relative efficiency in detecting Yersinia enterocolitica O:3 in naturally contaminated pork products. Method A was as described by Wauters et al. (Appl. Environ. Microbiol. 54:851-854, 1988). Method B has been recommended by the Nordic Committee on Food Analysis (method no. 117, 1987). The genetic probe was used in a colony hybridization assay to detect virulent yersiniae at each of the isolation steps with composed methods A and B. A total of 50 samples of raw pork products obtained from 13 meat-processing factories in Norway were examined. Y. enterocolitica serogroup O:3, biovar 4, was isolated from altogether 9 (18.0%) of the samples by using the two isolation procedures. In contrast, colony hybridization using the genetic probe indicated that 30 (60.0%) of the samples contained virulent yersiniae. All samples which were positive on cultivation were also positive by hybridization. The results indicate that the occurrence of pathogenic Y. enterocolitica in Norwegian pork products is substantially higher than previously demonstrated and, therefore, reinforce our suggestion that pork products represent an important potential source of human infection in Norway. The results also indicate that the use of conventional isolation procedures may lead to considerable underestimation of pathogenic Y. enterocolitica in pork products.

This article has been cited by other articles:

• Lambertz, S. T., Nilsson, C., Hallanvuo, S., Lindblad, M. (2008). Real-Time PCR Method for Detection of Pathogenic Yersinia enterocolitica in Food. Appl. Environ. Microbiol. 74: 6060-6067 [Abstract] [Full Text]  
• Falcao, J. P., Falcao, D. P., Pitondo-Silva, A., Malaspina, A. C., Brocchi, M. (2006). Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil.. J Med Microbiol 55: 1539-1548 [Abstract] [Full Text]  
• Fredriksson-Ahomaa, M., Korkeala, H. (2003). Low Occurrence of Pathogenic Yersinia enterocolitica in Clinical, Food, and Environmental Samples: a Methodological Problem. Clin. Microbiol. Rev. 16: 220-229 [Abstract] [Full Text]