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Appl Environ Microbiol. 1991 December; 57(12): 3405-3409
Copyright © 1991, American Society for Microbiology. All Rights Reserved.

Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40

The National Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Irish Republic

ABSTRACT

Lactococcus lactis subsp. lactis 425A is an atypical strain which excretes a high concentration of -acetolactate when grown in milk. The conjugative lactococcal plasmid pNP40, which encodes phage and nisin resistance, was introduced to strain 425A by conjugation, using resistance to phage and nisin as a selection. No phage-nisin resistance mutants were encountered. Transconjugants display complete resistance at both 21 and 39°C to those phage previously identified as lytic for 425A. Transconjugants lose their resistance characteristics when spontaneously cured of pNP40. The commercially important property of 425A—production of high levels of -acetolactic acid—is unaffected by the presence of pNP40.

^* Corresponding author.

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