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Appl Environ Microbiol. 1991 November; 57(11): 3331-3336

Fractionation of cellulases from the ruminal fungus Neocallimastix frontalis EB188.

Department of Animal Sciences, Washington State University, Pullman 99164-6332.

ABSTRACT

Cellulases from the ruminal fungus Neocallimastix frontalis EB188 were separated by using hydroxylapatite column chromatography. Seven carboxymethylcellulases, six avicelases, and four beta-glucosidases accounted for the majority of the activities. The separation of enzymes was confirmed by using polyacrylamide gel electrophoresis. Electrophoretic migration, analysis of hydrolysis products, and substrate specificity measurements suggested that several different cellulases were secreted in N. frontalis EB188. The possible relationship of cellulase diversity to protein glycosylation is discussed.