This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baya, A M Articles by Hetrick, F M Search for Related Content PubMed PubMed Citation Articles by Baya, A M Articles by Hetrick, F M Agricola Articles by Baya, A M Articles by Hetrick, F M

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 November; 57(11): 3114-3120

Biochemical and serological characterization of Carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish.

Department of Microbiology, University of Maryland, College Park 20742.

ABSTRACT

A comparative analysis of the phenotypic and serological properties of Carnobacterium strains associated with mortalities of cultured striped bass and channel catfish and the properties of isolates from wild brown bullhead catfish in the Chesapeake Bay area in Maryland was conducted. All of the strains were gram-positive, facultatively anaerobic, nonmotile, non-spore-forming rods occurring singly or in short chains. They did not produce cytochrome oxidase or catalase, did not reduce nitrate, failed to produce H2S, were unable to grow on acetate medium, and did not produce gas from glucose or gluconate. The temperature and salinity ranges for most of the strains were 10 to 37 degrees C and 0 to 6% NaCl, respectively. The strains all fermented mannitol and inulin and were arginine dihydrolase positive; these are typical characteristics of Carnobacterium piscicola. The carbohydrate fermentation pattern exhibited by all of the isolates with the API-50 CHL system was also very similar to that shown by C. piscicola. Acid was produced from ribose, glucose, fructose, mannose, mannitol, N-acetylglucosamine, amygdaline, arbutin, esculin, salicin, cellobiose, maltose, sucrose, trehalose, and gentiobiose. The Carnobacterium strains did not show proteolytic, lipolytic, amylolytic, or hemolytic activity. Eighteen drugs were tested; all strains proved to be resistant to chloramphenicol, gentamicin, kanamycin, streptomycin, trimethoprim, quinolones, and nitrofurans. The analysis of membrane proteins supported the phenotypic similarities, two main patterns were established, one shared by the striped bass isolates and the reference strain of C. piscicola and another shared by most of the catfish strains. However, the agglutination assays demonstrated that only one Carnobacterium strain from striped bass was serologically related to C. piscicola ATCC 35586.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

• Rachman, C., Kabadjova, P., Valcheva, R., Prevost, H., Dousset, X. (2004). Identification of Carnobacterium Species by Restriction Fragment Length Polymorphism of the 16S-23S rRNA Gene Intergenic Spacer Region and Species-Specific PCR. Appl. Environ. Microbiol. 70: 4468-4477 [Abstract] [Full Text]  
• Kabadjova, P., Dousset, X., Le Cam, V., Prevost, H. (2002). Differentiation of Closely Related Carnobacterium Food Isolates Based on 16S-23S Ribosomal DNA Intergenic Spacer Region Polymorphism. Appl. Environ. Microbiol. 68: 5358-5366 [Abstract] [Full Text]  
• Nilsson, L., Nielsen, M. K., Ng, Y., Gram, L. (2002). Role of Acetate in Production of an Autoinducible Class IIa Bacteriocin in Carnobacterium piscicola A9b. Appl. Environ. Microbiol. 68: 2251-2260 [Abstract] [Full Text]