This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vincent, M N Articles by Pierson, E A Search for Related Content PubMed PubMed Citation Articles by Vincent, M N Articles by Pierson, E A Agricola Articles by Vincent, M N Articles by Pierson, E A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 October; 57(10): 2928-2934

Genetic analysis of the antifungal activity of a soilborne Pseudomonas aureofaciens strain.

Monsanto Company, St. Louis, Missouri 63198.

ABSTRACT

Pseudomonas aureofaciens Q2-87 produces the antibiotic 2,4-diacetophloroglucinol (Phl), which inhibits Gaeumannomyces graminis var. tritici and other fungi in vitro. Strain Q2-87 also provides biological control of take-all, a root disease of wheat caused by this fungus. To assess the role of Phl in the antifungal activity of strain Q2-87, a genetic analysis of antibiotic production was conducted. Two mutants of Q2-87 with altered antifungal activity were isolated by site-directed mutagenesis with Tn5. One mutant, Q2-87::Tn5-1, did not inhibit G. graminis var. tritici in vitro and did not produce Phl. Two cosmids were isolated from a genomic library of the wild-type strain by probing with the mutant genomic fragment. Antifungal activity and Phl production were coordinately restored in Q2-87::Tn5-1 by complementation with either cosmid. Mobilization of one of these cosmids into two heterologous Pseudomonas strains conferred the ability to synthesize Phl and increased their activity against G. graminis var. tritici, Pythium ultimum, and Rhizoctonia solani in vitro. Subcloning and deletion analysis of these cosmids identified a 4.8-kb region which was necessary for Phl synthesis and antifungal activity.

This article has been cited by other articles:

• Mavrodi, O. V., Mavrodi, D. V., Weller, D. M., Thomashow, L. S. (2006). Role of ptsP, orfT, and sss Recombinase Genes in Root Colonization by Pseudomonas fluorescens Q8r1-96. Appl. Environ. Microbiol. 72: 7111-7122 [Abstract] [Full Text]  
• Rezzonico, F., Defago, G., Moenne-Loccoz, Y. (2004). Comparison of ATPase-Encoding Type III Secretion System hrcN Genes in Biocontrol Fluorescent Pseudomonads and in Phytopathogenic Proteobacteria. Appl. Environ. Microbiol. 70: 5119-5131 [Abstract] [Full Text]  
• Maurhofer, M., Baehler, E., Notz, R., Martinez, V., Keel, C. (2004). Cross Talk between 2,4-Diacetylphloroglucinol-Producing Biocontrol Pseudomonads on Wheat Roots. Appl. Environ. Microbiol. 70: 1990-1998 [Abstract] [Full Text]  
• Martinez, A., Kolvek, S. J., Yip, C. L. T., Hopke, J., Brown, K. A., MacNeil, I. A., Osburne, M. S. (2004). Genetically Modified Bacterial Strains and Novel Bacterial Artificial Chromosome Shuttle Vectors for Constructing Environmental Libraries and Detecting Heterologous Natural Products in Multiple Expression Hosts. Appl. Environ. Microbiol. 70: 2452-2463 [Abstract] [Full Text]  
• Duffy, B., Keel, C., Defago, G. (2004). Potential Role of Pathogen Signaling in Multitrophic Plant-Microbe Interactions Involved in Disease Protection. Appl. Environ. Microbiol. 70: 1836-1842 [Abstract] [Full Text]  
• Abbas, A., Morrissey, J. P., Marquez, P. C., Sheehan, M. M., Delany, I. R., O'Gara, F. (2002). Characterization of Interactions between the Transcriptional Repressor PhlF and Its Binding Site at the phlA Promoter in Pseudomonas fluorescens F113. J. Bacteriol. 184: 3008-3016 [Abstract] [Full Text]  
• McSpadden Gardener, B. B., Weller, D. M. (2001). Changes in Populations of Rhizosphere Bacteria Associated with Take-All Disease of Wheat. Appl. Environ. Microbiol. 67: 4414-4425 [Abstract] [Full Text]  
• Raaijmakers, J. M., Weller, D. M. (2001). Exploiting Genotypic Diversity of 2,4-Diacetylphloroglucinol-Producing Pseudomonas spp.: Characterization of Superior Root-Colonizing P. fluorescens Strain Q8r1-96. Appl. Environ. Microbiol. 67: 2545-2554 [Abstract] [Full Text]  
• McSpadden Gardener, B. B., Schroeder, K. L., Kalloger, S. E., Raaijmakers, J. M., Thomashow, L. S., Weller, D. M. (2000). Genotypic and Phenotypic Diversity of phlD-Containing Pseudomonas Strains Isolated from the Rhizosphere of Wheat. Appl. Environ. Microbiol. 66: 1939-1946 [Abstract] [Full Text]  
• Picard, C., Di Cello, F., Ventura, M., Fani, R., Guckert, A. (2000). Frequency and Biodiversity of 2,4-Diacetylphloroglucinol-Producing Bacteria Isolated from the Maize Rhizosphere at Different Stages of Plant Growth. Appl. Environ. Microbiol. 66: 948-955 [Abstract] [Full Text]  
• Delany, I., Sheehan, M. M., Fenton, A., Bardin, S., Aarons, S., O’Gara, F. (2000). Regulation of production of the antifungal metabolite 2,4-diacetylphloroglucinol in Pseudomonas fluorescens F113: genetic analysis of phlF as a transcriptional repressor. Microbiology 146: 537-546 [Abstract] [Full Text]  
• Bangera, M. G., Thomashow, L. S. (1999). Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. J. Bacteriol. 181: 3155-3163 [Abstract] [Full Text]