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Appl Environ Microbiol. 1991 October; 57(10): 2834-2840

Intermediates and products of synthetic lignin (dehydrogenative polymerizate) degradation by Phlebia tremellosa.

National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.

ABSTRACT

Agitated, nitrogen-limited cultures of Phlebia tremellosa caused substantial changes in the distribution of 14C-labelled synthetic lignin (dehydrogenative polymerizate [DHP]) between water-soluble, dioxane-soluble, alkali-soluble, and insoluble fractions before much lignin carbon was metabolized to CO2. First, the insoluble form increased at the expense of the dioxane-soluble form. Later, the amounts of alkali-soluble and water-soluble 14C increased, and release of 14CO2 began. The molecular weight distribution of the dioxane-soluble lignin remained constant during degradation, but that of the water-soluble fraction changed to higher molecular weights. Culture agitation accelerated the attachment of suspended DHP to the mycelia and stimulated production of water-soluble 14C and 14CO2. The nonionic detergent Tween 80 also hastened release of 14CO2 and increased the early conversion of dioxane-soluble DHP to the alkali-soluble and insoluble forms. Oxidative polymerization is suggested as the first step in degradation of DHP by P. tremellosa.

This article has been cited by other articles:

• Reid, I. D. (1998). Fate of Residual Lignin during Delignification of Kraft Pulp by Trametes versicolor. Appl. Environ. Microbiol. 64: 2117-2125 [Abstract] [Full Text]