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Appl Environ Microbiol. 1990 September; 56(9): 2930-2932

Detection of Listeria monocytogenes by using the polymerase chain reaction.

M T Bessesen, Q A Luo, H A Rotbart, M J Blaser and R T Ellison 3rd

Medical Service, Department of Veterans Affairs Medical Center, Denver, Colorado.

ABSTRACT

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.

Appl Environ Microbiol. 1990 September; 56(9): 2930-2932




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