This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuhn, E. P. Articles by Suflita, J. M. Search for Related Content PubMed PubMed Citation Articles by Kuhn, E. P. Articles by Suflita, J. M. Agricola Articles by Kuhn, E. P. Articles by Suflita, J. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1990 September; 56(9): 2630-2637
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Effect of Sulfate and Organic Carbon Supplements on Reductive Dehalogenation of Chloroanilines in Anaerobic Aquifer Slurries

Elmar P. Kuhn, G. Todd Townsend and Joseph M. Suflita^*

Department of Botany and Microbiology, The University of Oklahoma, 770 Van Vleet Oval, Norman, Oklahoma 73019

ABSTRACT

When di-, tri-, and tetrachloroaniline were incubated in methanogenic groundwater slurries, they were reductively dehalogenated by the aquifer microbiota. 2,3,4-Trichloroaniline was metabolized by two pathways. Primary dehalogenation occurred at either the meta or ortho position of this substrate to form 2,4- and 3,4-dichloroaniline, respectively. The latter chemical could be stoichiometrically converted to 3-chloroaniline. 2,3,4,5-Tetrachloroaniline was degraded by the sequential removal of halogens from the para and then the ortho position to form 3,5-dichloroaniline. An additional pathway was observed with this substrate when the aquifer slurries were amended with butyrate. That is, halogens could be removed from both the meta and ortho positions of tetrachloroaniline. The amendment of sulfate to methanogenic aquifer slurries slowed the rate of 2,3,4,5-tetrachloroaniline degradation and increased the amount of substrate channeled through the additional pathway. The reported intermediates or end products are identified by their chromatographic mobility and mass-spectral profiles.

^* Corresponding author.

Present address: Kantonales Laboratorium, CH-5000 Aarau, Switzerland.

Present address: Technical Resources Inc., U.S. Environmental Protection Agency, Gulf Breeze, FL 32561.

This article has been cited by other articles:

• Adrian, L., Manz, W., Szewzyk, U., Görisch, H. (1998). Physiological Characterization of a Bacterial Consortium Reductively Dechlorinating 1,2,3- and 1,2,4-Trichlorobenzene. Appl. Environ. Microbiol. 64: 496-503 [Abstract] [Full Text]