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Appl Environ Microbiol. 1990 August; 56(8): 2562-2564

Isolation of Candida tropicalis auxotrophic mutants.

M A Gleeson, L O Haas and J M Cregg

Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, California 92037.

ABSTRACT

An enrichment scheme using nystatin was designed for the isolation of auxotrophic mutants from the diploid-alkane-utilizing yeast Candida tropicalis. A collection of 194 auxotrophs representing 7 phenotypes was isolated. One class of mutants was identified as having a defect in histidinol dehydrogenase activity and a second class of mutants was identified as having a defect in orotidine monophosphate decarboxylase activity. These strains are good candidates to be carrying mutations corresponding to the HIS4 and URA3 genes of Saccharomyces cerevisiae.

Appl Environ Microbiol. 1990 August; 56(8): 2562-2564






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