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Appl Environ Microbiol. 1990 August; 56(8): 2517-2521
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Effects of Culture Conditions on Poly(ß-Hydroxybutyric Acid) Production by Haloferax mediterranei

Departamento de Genética Molecular y Microbiología, Campus de San Juan, Apdo. 374, Universidad de Alicante, 03080 Alicante, Spain

ABSTRACT

The halobacterium Haloferax mediterranei accumulates poly(ß-hydroxybutyrate) (PHB) as intracellular granules. The conditions for PHB production in batch and continuous cultures have been studied and optimized. Phosphate limitation is essential for PHB accumulation in large quantities. Glucose and starch are the best carbon sources. With 2% starch, 0.00375% KH₂PO₄, and 0.2% NH₄Cl in batch culture, a production of ca. 6 g of PHB per liter was reached, being 60% of the total biomass dry weight, and giving a yield over the carbon source of 0.33 g/g. The PHB production in continuous cultures was stable over a 3-month period. Our results demonstrate that H. mediterranei is an interesting candidate for industrial production of biological polyesters.

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