This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Breedveld, M W Articles by Zehnder, A J Search for Related Content PubMed PubMed Citation Articles by Breedveld, M W Articles by Zehnder, A J Agricola Articles by Breedveld, M W Articles by Zehnder, A J

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1990 July; 56(7): 2080-2086

Excessive excretion of cyclic beta-(1,2)-glucan by Rhizobium trifolii TA-1.

Department of Microbiology, Agricultural University, Wageningen, The Netherlands.

ABSTRACT

At 25 degrees C, the optimal temperature for growth of Rhizobium trifolii TA-1, extracellular and capsular polysaccharide (EPS and CPS) were the main carbohydrate products synthesized in mannitol-rich medium (10 g of mannitol and 1 g of glutamic acid per liter). In the same medium at 33 degrees C, EPS and CPS production was inhibited, and up to 3.9 g of cyclic beta-(1,2)-glucan was produced during an incubation period of 20 days with a total biomass of 0.55 g of protein. In a medium containing 50 g of mannitol and 10 g of glutamic acid per liter, high cell densities (3.95 g of protein) were obtained at 25 degrees C. This biomass excreted 10.9 g of cyclic beta-(1,2)-glucan within 10 days. Concomitantly, 4.8 g of EPS were synthesized, while CPS production was strongly suppressed. The excreted cyclic beta-(1,2)-glucans were neutral and had degrees of polymerization ranging from 17 to 25, with a degree of polymerization of 19 as the major glucan cycle.

This article has been cited by other articles:

• Laus, M. C., Logman, T. J., van Brussel, A. A. N., Carlson, R. W., Azadi, P., Gao, M.-Y., Kijne, J. W. (2004). Involvement of exo5 in Production of Surface Polysaccharides in Rhizobium leguminosarum and Its Role in Nodulation of Vicia sativa subsp. nigra. J. Bacteriol. 186: 6617-6625 [Abstract] [Full Text]